Although phage display is a favorite technique for the identification of antigen-specific antibodies from immune libraries, it is also well known that it is a biased methodology: only well-expressed clones this system are selected. It has been demonstrated that clones missed by phage display but identified by repertoire mining may exhibit higher affinities.
Abquery is a simple, easy and fast tool for repertoire mining. A query antibody sequences are used as a bait for finding homologs in NGS repertoire. The in-silico identified clones are then synthesized and tested or used as a template in antibody in-vitro maturation processes.
Abquery mines the repertoire and finds sequences variants and display the results as multiple alignments with statistical data.
Abquery finds similar VH sequences. Abaligner aligns the sequences hits with the query VH as a consensus template sequence. Mutations are highlighted in the alignment and Wu-Kabat variability and AA position-frequency data generated.